[DESeq2] Time-Course Experimental design

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6.8 years ago

inzirio • 0

I'd like to perform an experimental design for DESeq2 package using the LRT Test.

I've already done it before using two different conditions Treated (T) vs Untreated (U), each at different time points.

I was wondering how to design an experiment having the U replicates just at time point 0 and T replicated samples at different time-points.

Is it possible to perform it with DESeq2? I'm not able to find examples as in this special case, but i'm able to find several time-course data designed in this way.

Thanks, Inzirio

RNA-Seq R time-course • 2.3k views

ADD COMMENT • link updated 6.8 years ago by badribio ▴ 290 • written 6.8 years ago by inzirio • 0

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crosspost https://support.bioconductor.org/p/98424/

ADD REPLY • link 6.8 years ago by badribio ▴ 290

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Hello mioappoggio!

It appears that your post has been cross-posted to another site: https://support.bioconductor.org/p/98424/ (and answered there)

This is typically not recommended as it runs the risk of annoying people in both communities.

ADD REPLY • link 6.8 years ago by WouterDeCoster 47k

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