Hi, I am beginner in this area and I am not able to resolve the option -subject in blast. It seems that it represents sequences which are compared to query sequences, but how it differs from database then?
BLAST is an acronym for Basic Local Alignment Search Tool and refers
to a suite of programs used to generate alignments between a
nucleotide or protein sequence, referred to as a “query” and
nucleotide or protein sequences within a database, referred to as