Entering edit mode
6.7 years ago
prathyushakonda
▴
20
Hi, we are planning to do chirp-seq for a lncRNA of interest. The company uses HiSeq2000 for this. We are wondering about what sequencing depth is ideal for this. Would 100 mil. reads be sufficient enough to get good coverage? I would greatly appreciate your valuable insights.
In my opinion, 100M should be very good. You could check ChRiP papers and see what was their sequencing depth.