Question: Viral Whole genome Deep Sequencing within Human Host
0
gravatar for mhmtgenc85
3.5 years ago by
mhmtgenc8540
Turkey
mhmtgenc8540 wrote:

Dear All,

I have a question about whole genome sequencing of a virus. There is a viral sample but in a serum of human sample And would like to have deep sequencing to assemble the whole genome of this virus. So what should be the coverage of mine? Could you please give me an opinion. By the way the virus is Parvovirus.

Thanks in advance

ADD COMMENTlink written 3.5 years ago by mhmtgenc8540

What is the expected genome size for the virus? Are you able to enrich the viral sequences in any way to have better chance of sequencing them?

ADD REPLYlink modified 3.5 years ago • written 3.5 years ago by GenoMax95k

the expected genome size for the virus is around 5-6kb. And do you have any idea how should I have a chance to enrich the viral sequences?

ADD REPLYlink written 3.5 years ago by mhmtgenc8540

Oligonucleotide probes for target capture or PCR based amplification.

ADD REPLYlink written 3.5 years ago by WouterDeCoster45k
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