Hi all, I have a question about gene expression. I mapped RNA-seq reads back to the genome and noticed there are my expression peaks within a gene. Does it cause by library preparation process, sequencing problem, or a real biological thing? The blue bar on the top is a gene(single-exon gene), the red below is bam file generated by mapping reads. Has anyone got the same problem?
I uploaded the figure of a gene expression pattern on my GitHub, here is the link. Hope it works. I was thinking if there is a way to make uploading figure easier?