NEB Vs ScriptSeq
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6.7 years ago
poojasethiya ▴ 120

I have two RNASeq libraries ( (i.e. NEB and ScriptSeq) of same sample. I have used same pipeline to align the reads and perform further down stream analysis. But upon comparison of DEG, I get different results. About 60% of the genes UP-regulated in NEB overlap with DOWN genes in NEB. What can be the probable reason?

Thank you in advance.

RNA-Seq alignment Assembly • 1.1k views
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