data set geneList not found, problem in the topGO tutorial
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4.3 years ago
cristian ▴ 300

Hi,

I am trying to do some GO term enrichment analysis and am following this tutorial:

http://bioconductor.org/packages/release/bioc/vignettes/topGO/inst/doc/topGO.pdf

> library(topGO)
Loading required package: GO.db
Loading required package: AnnotationDbi
Error in value[[3L]](cond) : 
  Package ‘AnnotationDbi’ version 1.38.1 cannot be unloaded:
 Error in unloadNamespace(package) : namespace ‘AnnotationDbi’ is imported by ‘GenomicFeatures’, ‘genefilter’, ‘biomaRt’, ‘VariantAnnotation’, ‘GO.db’, ‘annotate’, ‘geneplotter’, ‘topGO’ so cannot be unloaded
> library(ALL)
> data(ALL)
> data(geneList)
Warning message:
In data(geneList) : data set ‘geneList’ not found

As you can see, I am encountering problems. Can anybody advise me on what to do to get ahead, please? I don't understand why topGO needs to be unloaded. Surely, it should be loaded.

Thanks.

Best,

C.

go functional genomics bioconductor ALL topgo • 2.3k views
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1
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Try re-installing topGO. I have no issues with your code.

Also, please post the result of sessionInfo().

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    > biocLite('topGO')
    BioC_mirror: https://bioconductor.org
    Using Bioconductor 3.5 (BiocInstaller 1.26.0), R 3.4.0 (2017-04-21).
    Installing package(s) ‘topGO’
    trying URL 'https://bioconductor.org/packages/3.5/bioc/src/contrib/topGO_2.28.0.tar.gz'
    Content type 'application/x-gzip' length 1262288 bytes (1.2 MB)
    ==================================================
    downloaded 1.2 MB

    * installing *source* package ‘topGO’ ...
    ** R
    ** data
    ** inst
    ** preparing package for lazy loading
    ** help
    *** installing help indices
    ** building package indices
    ** installing vignettes
    ** testing if installed package can be loaded

    groupGOTerms:   GOBPTerm, GOMFTerm, GOCCTerm environments built.
    * DONE (topGO)

    The downloaded source packages are in
        ‘/private/var/folders/z2/rt6qxntd3tg5bjmqlqnm0mdm0000gn/T/RtmpRkxLWl/downloaded_packages’


> library(topGO)
Loading required package: GO.db
Loading required package: AnnotationDbi
Error in value[[3L]](cond) : 
  Package ‘AnnotationDbi’ version 1.38.1 cannot be unloaded:
 Error in unloadNamespace(package) : namespace ‘AnnotationDbi’ is imported by ‘GenomicFeatures’, ‘genefilter’, ‘biomaRt’, ‘VariantAnnotation’, ‘GO.db’, ‘annotate’, ‘geneplotter’, ‘topGO’ so cannot be unloaded
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sessionInfo() R version 3.4.0 (2017-04-21) Platform: x86_64-apple-darwin16.5.0 (64-bit) Running under: macOS Sierra 10.12.5

Matrix products: default
BLAS: /System/Library/Frameworks/Accelerate.framework/Versions/A/Frameworks/vecLib.framework/Versions/A/libBLAS.dylib
LAPACK: /System/Library/Frameworks/Accelerate.framework/Versions/A/Frameworks/vecLib.framework/Versions/A/libLAPACK.dylib

locale:
[1] en_GB.UTF-8/en_GB.UTF-8/en_GB.UTF-8/C/en_GB.UTF-8/en_GB.UTF-8

attached base packages:
[1] stats4    parallel  stats     graphics  grDevices utils     datasets 
[8] methods   base     

other attached packages:
[1] ALL_1.18.0           IRanges_2.10.2       S4Vectors_0.14.3    
[4] Biobase_2.36.2       graph_1.54.0         BiocGenerics_0.22.0 
[7] BiocInstaller_1.26.0 bindrcpp_0.2         dplyr_0.7.2         

loaded via a namespace (and not attached):
  [1] TH.data_1.0-8              colorspace_1.3-2          
  [3] seqinr_3.4-5               rprojroot_1.2             
  [5] htmlTable_1.9              XVector_0.16.0            
  [7] GenomicRanges_1.28.4       base64enc_0.1-3           
  [9] topGO_2.28.0               bit64_0.9-7               
 [11] AnnotationDbi_1.38.2       mvtnorm_1.0-6             
 [13] codetools_0.2-15           splines_3.4.0             
 [15] tximport_1.4.0             doBy_4.5-15               
 [17] geneplotter_1.54.0         knitr_1.16                
 [19] ade4_1.7-6                 Formula_1.2-2             
 [21] jsonlite_1.5               Rsamtools_1.28.0          
 [23] annotate_1.54.0            GO.db_3.4.1               
 [25] cluster_2.0.6              pheatmap_1.0.8            
 [27] compiler_3.4.0             backports_1.1.0           
 [29] assertthat_0.2.0           Matrix_1.2-10             
 [31] lazyeval_0.2.0             acepack_1.4.1             
 [33] htmltools_0.3.6            tools_3.4.0               
 [35] gtable_0.2.0               glue_1.1.1                
 [37] GenomeInfoDbData_0.99.0    Rcpp_0.12.12              
 [39] Biostrings_2.44.2          ape_4.1                   
 [41] nlme_3.1-131               rtracklayer_1.36.4        
 [43] pinfsc50_1.1.0             refGenome_1.7.3           
 [45] stringr_1.2.0              network_1.13.0            
 [47] devtools_1.13.3            XML_3.98-1.9              
 [49] zlibbioc_1.22.0            MASS_7.3-47               
 [51] zoo_1.8-0                  scales_0.4.1              
 [53] microbenchmark_1.4-2.1     BSgenome_1.44.0           
 [55] VariantAnnotation_1.22.3   SummarizedExperiment_1.6.3
 [57] sandwich_2.4-0             SparseM_1.77              
 [59] RColorBrewer_1.1-2         yaml_2.1.14               
 [61] memoise_1.1.0              gridExtra_2.2.1           
 [63] ggplot2_2.2.1              biomaRt_2.32.1            
 [65] rpart_4.1-11               latticeExtra_0.6-28       
 [67] stringi_1.1.5              RSQLite_2.0               
 [69] genefilter_1.58.1          checkmate_1.8.3           
 [71] permute_0.9-4              GenomicFeatures_1.28.4    
 [73] BiocParallel_1.10.1        GenomeInfoDb_1.12.2       
 [75] rlang_0.1.1                pkgconfig_2.0.1           
 [77] matrixStats_0.52.2         bitops_1.0-6              
 [79] evaluate_0.10.1            lattice_0.20-35           
 [81] bindr_0.1                  GenomicAlignments_1.12.1  
 [83] htmlwidgets_0.9            labeling_0.3              
 [85] bit_1.1-12                 plyr_1.8.4                
 [87] magrittr_1.5               DESeq2_1.16.1             
 [89] R6_2.2.2                   Hmisc_4.0-3               
 [91] multcomp_1.4-6             DelayedArray_0.2.7        
 [93] DBI_0.7                    foreign_0.8-69            
 [95] withr_2.0.0                mgcv_1.8-18               
 [97] survival_2.41-3            RCurl_1.95-4.8            
 [99] nnet_7.3-12                tibble_1.3.3              
[101] vcfR_1.5.0                 rmarkdown_1.6             
[103] locfit_1.5-9.1             grid_3.4.0                
[105] data.table_1.10.4          blob_1.1.0                
[107] vegan_2.4-3                digest_0.6.12             
[109] xtable_1.8-2               munsell_0.4.3             
[111] viridisLite_0.2.0          gsalib_2.1
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