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6.7 years ago
Halcyon Murphy
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0
Greetings, everyone.
I am just new to this field and looking for help since the lab majors in medicinal chemistry.
We got mRNA-Seq data of our cells from other lab. The data we have only gives FPKM associated with gene names and subjects. The PI wants me to find out the biomarkers among these cells from mRNA data.
According to the knowledge and references I have, packages from bioconductor are mostly used for this kind of analysis. However, the ones often used like GOSeq, edgeR, DESeq2 require raw data, such as count number from reading. Is there any suggestion what I should use or how I should do?
Thanks for attentions and help.
PS: I cannot get access to the count per million (CPM) or other data. The table with FPKM is all I have.
