When I read some papers which have used third sequencing technology, e.g. PacBio iso-seq, I didn't find any paper which use the long reads to measure gene expression level. They did RNA-seq by third sequencing, but measured gene expression level by second sequencing short reads. Can someone tell me why or give an example using third sequencing long reads to measure the gene expression level? Is it possible to use the third sequencing long reads to estimate gene expression level?
As far as I know, PacBio sequencing is not quantitative. The main aim of ISO-Seq is to to sequence end to end transcripts, to identify the novel transcripts and to get the full length transcript structure. Its qualitative but not quantitative. For quantitative RNA-Seq, you need to use illumina "like" sequencing platforms.