expression to networking
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4.8 years ago
krushnach80 ★ 1.0k

I would like to know how to proceed from my gene expression data to building a network from that expression data .

So far what im doing is checking the differential expression of genes and see how it changes ..but that wont give me a global picture i suppose ..So it would be helpful if anyone can suggest me how do i proceed what kind of approach should i take .Suggestion would be really appreciable

RNA-Seq • 1.4k views
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First: Get the list of genes that are most significant deferentially expressed using a statistical package like DESEQ2

Second: Pass the gene list to StringDB

Third: Import the output of StringDB to Cytoscape

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so i wont be doing any ranking of deferentially expressed genes which i have from Deseq2 , i could just put the huge list to string DB? lets say i have 1000 diff expressed genes , would it be meaningful to pass the gene list to string DB?

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Well the basic principle of plotting a network of related genes is that you must try to plot a network of RELATED genes. So rather than passing a RANDOM gene set we first make a set of genes with something in COMMON (e.g. a differentially expressed gene set, or co-expression gene set, or tissue specific gene set etc. ) in the hope that a biologically meaningful network can be constructed.

The key word here...is HOPE.

I would rank the significantly differentially expressed genes by padj and select then the top 1000. Hopefully you will get some nice networks in there. But expect to see lots of unconnected genes.

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4.8 years ago
aka001 ▴ 190

That would depend on your experimental design and what kind of biological questions you have in mind, I would say.

You can check BioLayout 3D package (http://www.biolayout.org/) and check their tutorials. You can build some network together with the visualization through that package and I also found their tutorials really helpful.

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Biolayout 3D has been deprecated and is no longer supported..Miru (https://kajeka.com/) is its successor and is faster and more optimised than its predecessor..

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4.8 years ago
Corentin ▴ 490

If you have access to the enzyme annotations for your genes, you can use the KEGG API which can link enzymes to kegg pathways (http://www.kegg.jp/kegg/rest/keggapi.html). You can use R (or your favorite language) to access the API.

The file to link enzymes and pathways is available here : http://rest.kegg.jp/link/pathway/enzyme.

The advantage of this approach is that you can color your enzymes according to up-down regulation.

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4.7 years ago
xanderpico ▴ 450

Perhaps a bit late, but given a single differential expression result, I typically go to a resource like GeneMANIA to generate a network based on more comprehensive co-expression analysis and then import that into Cytoscape. Then I overlay my diff exp data onto that network to assess the "functional" modules being regulated in my dataset.

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4.8 years ago
mforde84 ★ 1.4k

WGCNA comes to mind. Though coexpression networks need a lot of samples to be accurate.

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well yes....but apart from that ?anything i have this flowchart can you give me more insight flowchart

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There's like 40+ pages of documentation on WGCNA... You can do it! I have faith in you!

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