Question: Design qpcr primers
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gravatar for sbchua.1990
2.5 years ago by
sbchua.199030
sbchua.199030 wrote:

Hi, I did RNA-seq experiment and now I wish to validate my data using real time qPCR.

Any suggestion for softwares and pipeline for primers design? How to design primers that span exon-exon junction?

qpcr rna-seq sequence primers • 929 views
ADD COMMENTlink modified 2.5 years ago by James Ashmore2.8k • written 2.5 years ago by sbchua.199030
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gravatar for James Ashmore
2.5 years ago by
James Ashmore2.8k
UK/Edinburgh/MRC Centre for Regenerative Medicine
James Ashmore2.8k wrote:

If you need to design a small number of primers then use an online web tool such as Primer-BLAST, however if you have need to design hundreds then it's probably easier creating your own primer design pipeline from various open source tools. Right now, I'm using the command-line version of primer3 to design my primers, and then using primersearch to make sure they are unique.

ADD COMMENTlink written 2.5 years ago by James Ashmore2.8k
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