Entering edit mode
6.7 years ago
mail2steff
▴
70
Hello,
I am using MISO for studying alternative splicing sites in Arabidopsis thaliana. MISO needs different types of annotation files namely: Exon-centric annotations, Isoform-centric annotations and Alternative 3’ UTR annotations.
I do not know from where I can download these annotation files for Arabidopsis thaliana. But I tried to index with the gff3 file downloaded from ensembl plants. But when I execute further commands I am getting 0 events:
summarize_miso --summarize-samples my_output1/ summaries/
MISO (Mixture of Isoforms model)
Summarize MISO output to get Psi values and confidence intervals.
Use --help argument to view options.
Loading events from: Splicing_events/Sequences/my_output1
Writing summary to: Splicing_events/Sequences/summaries/summary/my_output1.miso_summary
- Summarized a total of 0 events.
[64]% compare_miso --compare-samples my_output1/ my_output2/ comparison_output/
Making comparisons directory: /Splicing_events/Sequences/comparison_output
Given output dir: Splicing_events/Sequences/comparison_output
Retrieving MISO files in sample directories...
Computing sample comparison between Splicing_events/Sequences/my_output1 and /Splicing_events/Sequences/my_output2...
- No. of events in/Splicing_events/Sequences/my_output1: 0
- No. of events in /Splicing_events/Sequences/my_output2: 0
Creating comparisons parent directory:/Splicing_events/Sequences/comparison_output/my_output1_vs_my_output2
Compared a total of 0 events.
Can anyone guide me ? Thank you