I am trying to understand the HG-U95 chip microarray expression data which is using in the GSEA. I have understood the parameter in the p53 data set. It has 50 columns, first 17 columns are expressed as WT(Wild Type) and other 33 columns are expressed as MUT(Mutation). Rows are represented as no of features (genes) and values are represented as the expression values. But I have following questions about the parameters and values in that dataset.
- How is this dataset differentially expressed as WT and MUT?
- What is the biological meaning of this expression value?