Question: tblastx returning hits with 33% query cover for human genes against the human genome
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2.0 years ago by
Jacob10
Jacob10 wrote:

I'm running command line tblastx for some human genes as the query sequences against the human genome as the target sequence. Almost all the searches are returning a query cover (qcovhsp) of 33%. I'm thinking that it is no coincidence that amino acids are 3 nucleotides long and that my query covers are ~ 1/3. Can anyone give me some insight as to why these values are being returned and not 100, as is returned when I use blastn?

blast tblastx query cover • 541 views
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