Hi, when proofreading for a colleague I just stumbled upon an unreferenced claim that we have thousands to millions of sequence copies in each cluster using Illumina technology.
I found another article from 2008 claiming "approximatly 1000".
But I can't imagine this numbers are still accurate for current technology including two-colour chemistry. If the amplification is not restricted too much by reagents or binding sites on the flowcell, 35 cycles of cluster generation for v4 Chemistry should generate a lot more.
Does anybody know the numbers or have a reference for current technology systems/flowcells/chemistry?