Question: Is there any significant difference between endogenous/ native promoter and constitutive promoter (35S) on protein abundance?
gravatar for Wuschel
2.3 years ago by
Wuschel270 wrote:

If a same gene is clone either endogenous promoter or constitutive promoter (35S), Do we get a significantly different protein abundance?

ADD COMMENTlink modified 2.3 years ago by Jean-Karim Heriche21k • written 2.3 years ago by Wuschel270

This is not a bioinformatics question and would be more appropriate to biology.stackexchange.

ADD REPLYlink written 2.3 years ago by Jean-Karim Heriche21k
gravatar for Jean-Karim Heriche
2.3 years ago by
EMBL Heidelberg, Germany
Jean-Karim Heriche21k wrote:

This outcome is unpredictable as far as I know. This would depend on the relative "strength" of the two promoters, whether and how they would be regulated in the cell you're using. The type of expression/overexpression you're using is also a factor, e.g. if doing stable transfection with random integration in the genome, your clone will be subject to regulation by the chromatin environment it ends up in.

ADD COMMENTlink written 2.3 years ago by Jean-Karim Heriche21k
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