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Question: Is there any significant difference between endogenous/ native promoter and constitutive promoter (35S) on protein abundance?
1
gravatar for Wox
3.0 years ago by
Wox • 340
HUJI
Wox • 340 wrote:

If a same gene is clone either endogenous promoter or constitutive promoter (35S), Do we get a significantly different protein abundance?

promoter gene expression genome • 980 views
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modified 3.0 years ago by Jean-Karim Heriche ♦ 23k • written 3.0 years ago by Wox • 340

This is not a bioinformatics question and would be more appropriate to biology.stackexchange.

ADD REPLY • link written 3.0 years ago by Jean-Karim Heriche ♦ 23k
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gravatar for Jean-Karim Heriche
3.0 years ago by
Jean-Karim Heriche ♦ 23k
EMBL Heidelberg, Germany
Jean-Karim Heriche ♦ 23k wrote:

This outcome is unpredictable as far as I know. This would depend on the relative "strength" of the two promoters, whether and how they would be regulated in the cell you're using. The type of expression/overexpression you're using is also a factor, e.g. if doing stable transfection with random integration in the genome, your clone will be subject to regulation by the chromatin environment it ends up in.
ADD COMMENT • link written 3.0 years ago by Jean-Karim Heriche ♦ 23k
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