Latest
Open
RNA-Seq
ChIP-Seq
SNP
Assembly
Tutorials
Tools
Jobs
Forum
Planet
All »
Home
Log In
Question: Is there any significant difference between endogenous/ native promoter and constitutive promoter (35S) on protein abundance?
1
gravatar for Wuschel
19 months ago by
Wuschel • 120
HUJI
Wuschel • 120 wrote:

If a same gene is clone either endogenous promoter or constitutive promoter (35S), Do we get a significantly different protein abundance?

promoter gene expression genome • 556 views
ADD COMMENT • link •
modified 19 months ago by Jean-Karim Heriche ♦ 18k • written 19 months ago by Wuschel • 120

This is not a bioinformatics question and would be more appropriate to biology.stackexchange.

ADD REPLY • link written 19 months ago by Jean-Karim Heriche ♦ 18k
0
gravatar for Jean-Karim Heriche
19 months ago by
Jean-Karim Heriche ♦ 18k
EMBL Heidelberg, Germany
Jean-Karim Heriche ♦ 18k wrote:

This outcome is unpredictable as far as I know. This would depend on the relative "strength" of the two promoters, whether and how they would be regulated in the cell you're using. The type of expression/overexpression you're using is also a factor, e.g. if doing stable transfection with random integration in the genome, your clone will be subject to regulation by the chromatin environment it ends up in.
ADD COMMENT • link written 19 months ago by Jean-Karim Heriche ♦ 18k
Please log in to add an answer.

Similar posts • Search »

Content
Help
Access
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1764 users visited in the last hour