I have a protein sequence and i need to mutate a sequence without changing the folds. Is there any tool available to check which amino acid residues in protein sequence can be mutated?
Please edit your question to contain enough detail to answer it. What do you mean by: "mutate a sequence without changing the folds". I guess you mean "which residues can be mutated without changing the structure of the protein (too much)"? What is your application of knowing these unconstrained residues?
I think he wants to get a list of possible SNVs at a position from the given codon in the cDNA so he doesnt have to go through the ones that aren't biologically possible for an SNV.
Like all synonymous codons? Certainly, that would keep the protein sequence identical.
Btw.: If it's this I would use a chain of EMBOSS tools: transeq | backtranambig, that should output all ambiguous nucleotide sequences. However, if the application to express recombinant protein, it would be better to use backtranseq with a proper codon-frequency table.
transeq | backtranambig
Which protein are you interested in? I have couple graphs where you can check that, if I have the one you are looking for maybe I can help
oph is the protein sequence
Sphingopyxis sp. (strain 113P3) with Uniprot ID of Q588Z2 ?