Cannot calculate order statistic on object with 3 columns in R

Question

[user_g]

I have 3 .CEL files which are replicates. Im trying to get the diffrentially expressed genes between them but when I reach this line : "celfiles.filtered <- nsFilter(celfiles.normalise, require.entrez=TRUE, remove.dupEntrez=TRUE)"

I get this error: " Error in rowQ(eSet, floor(0.25 * numSamp)) : cannot calculate order statistic on object with 3 columns".

I'm trying to filter the probes after normalizing them to proceed to analyze them using statistical values.

I tried searching for this error but I couldn't reach anywhere so please any help.

[cpad0112]

what does celfiles.normalise contain and what is class and structure of celfiles.normalise?

[Matteo Schiavinato]

Perhaps you should only have row.names and values, for each file? How are your files composed, what do the columns contain?