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• Comparing two RNA-seq datasets of same species and treatments
  I have RNA-seq studies from two labs that did similar experiments (used same species and both use...
• Depth of Single Cell RNA seq experiment
  Hello, I am new to this field, and would greatly appreciate an answer to this question. I am l...
• Scatter plot for two data sets
  Hello every one I have two CSV files from different RNA seq data, I like to do scatter plot to ...
• RNA-seq analysis of two species
  Dear all, I have RNA-seq samples of two related species with similar genomes. The quality of gen...
• how to normalise/Scale the FPKM and Ka/Ks value in a matrix?
  I've got two columns about RNA-seq FPKM between two species and a ka/ks column of these ortholog,...
• Public data access: Single-cell RNA-seq and Buck RNA-seq data
  Hey colleagues,  Is there any public dataset which contains single-cell RNA-seq and Bulk RNA-seq...
• qulity control of RNA-seq results
  Hi, Should be a correlation between number of good reads and RNA concentrations in the result of...
• RNA-Seq: does stranded and directional mean the same thing?
  I'm confused about the difference between the two terms. It seems to me that they mean the same t...
• How To Analyse Rna-Seq Biological Replicates
  I have two replicates, how to analyse RNA-seq data between the two replicates? Can I just add th...
• why dont we need technical replicate in case of RNA Seq.?
  I want to do RNA seq comparison between two situation in case of plant. is it necessary to use te...
• Quantifying repetitive elements in RNA-seqs
  Hello, Could you please give an almost easy quantification method suggestion for repetitive elem...
• How to make a PCA plot to check similar samples between two cohorts?
  I have 20 samples with gene intensities from Microarrays and same 20 samples with counts from RNA...
• How to analyse RNA-SEQ duplicated data coming from a low cluster denstiy experiment
  Hello all, I have recently received data from a RNA-Seq experiment. Apparently there has been a ...
• Difference Between PolyA and Hybrid Selection for RNA-Seq
  Hey all, I'm working with a data set from dbGaP through NCBI, and I'm looking to download some R...
• Selecting and de-selecting exon-exon junctions based on depth or coverage from RNA-seq mapping
  In RNA-seq mapping with TopHat, the exon-exon junctions are reported in junction.bed file. This i...
• Rna Extract In Rna-Seq Experiments
  Hello, It is often the case for the RNA extract in RNA-seq experiments to be either Long PolyA+ ...
• Finding the minimum distance between any two genes in a list / Coping with pseudo-genes in RNA-Seq data?
  Context: Sometimes, I get badly mapped RNA-Seq reads due to pseudo-genes (here close duplicates w...
• 2D Rna Structure For Proteins.
  Hi everyone I have two datasets of proteins, Secreted and cytoplasmic.Each set includes at least...
• FPKM values from SE vs. PE RNA-Seq libraries
  Hi everyone, I have a question regarding the use of RSEM for estimating isoform expression leve...
• RNA-seq DESeq2 : p-values and venn plots in same analysis
  I am doing differential expression analysis. I am comparing two different experiments, each exper...
• Strand specific and unstranded dataset
  Hey everybody, I am working with an RNA-seq dataset that combines samples from strand-specific a...