can i use any rna-seq dataset for viral quasispecies estimation?

Question

[rajeshkumar_vinod]

Hello,

I know there is specific method for viral quasispecies estimation called CirSeq. As compared to standard RNA-seq protocol CirSeq data contain less error rate.

Can a RNA-seq dataset generated using standard protocols be used for viral quasispecies estimation?

If yes, what should be the read depth?

Thank You.