Entering edit mode
6.6 years ago
andreiareis1987
▴
40
Hi guys,
I am "remaking" some tasks (with updated data) from a paper and one of them is get alternative splicing frequency for some GO categories but i am stuck i read again and again the paper and i don't figure out how they got the numbers ... and asking for help to the writer not good ideia because the paper is from 2007. :/ Please someone help me to get frequency, %, count of alternative splicing for each isoform from homo sapiens.
Thanks in advance.
As we have no idea of what kind of data you have and what you made so far, it is very difficult to provide any suggestions. Please read How To Ask Good Questions On Technical And Scientific Forums.
Sorry about my lack of clearness.
My data is from ensembl: homo sapiens version 89.38. I downloaded the gff file for all genome to get the specific cellular GO ids according to the paper https://bmcevolbiol.biomedcentral.com/articles/10.1186/1471-2148-7-188 that i am reviewing.
For the alternative splicing i am thinking about go to the ncbi and filter the genes that are annotated as alternative spliced and some how get the %AS...