Question: sense versus antisense (plus or minus) sequence alignment
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gravatar for a.alsayed.phd
11 weeks ago by
a.alsayed.phd0 wrote:

Please, if I have a couple of query (parts of virus genome) sequences and a couple of references sequences, what are the steps required to align them correctly;i.e, to ensure that I alighn sense with sense etc. (the same direction)?

Many Thanks

ADD COMMENTlink modified 10 weeks ago • written 11 weeks ago by a.alsayed.phd0
1

What are exactly "parts of virus genome"? RNAseq reads? An assembled virus transcriptome? Could you provide more details?

ADD REPLYlink written 10 weeks ago by h.mon9.2k

DNA seqs reads - sanger sequencing of PCR products

viral capsid regions (VPs) of human rhinoviruses

ADD REPLYlink written 10 weeks ago by a.alsayed.phd0

All sequence aligners check both orientations when doing alignments.

ADD REPLYlink written 10 weeks ago by genomax37k
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