Question: Do I have to compared sequenced miRNA reads against bovine miRNA from miRBase mature.fa sequence or entire bovine genome?
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2.7 years ago by
United States
bioinforesearchquestions280 wrote:

Hello friends,

I have sequenced reads from bovine miRNA (single end, raw reads 51bp). I have two groups. Each group with 4 repeats.

For example:

  • GroupA (GroupA_1, GroupA_2, GroupA_3, GroupA_4)

  • GroupB (GroupB_1, GroupB_2, GroupB_3, GroupB_4)

After trimming, I retained the reads with length 18-35 bases. My aim is to identify differential miRNA expression profiles.

Do I have to compared sequenced miRNA reads against bovine miRNA from miRBase mature.fa sequence or entire bovine genome?

Then planning to use htseq-count to compute the counts for Bovine miRNAs.

mirna rna-seq small rna • 654 views
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