fastQC for Oxford Nanopore reads
2
3
Entering edit mode
6.6 years ago
efratk ▴ 40

Hi,

I want to use fastQC for quality control checks on nanopore sequencing reads. When I ran fastQC it ran a long time without finishing or given an error. I stopped the job after several hours and got an error: "java.lang.OutOfMemoryError: Java heap space" What is the reason for it? Is fastQC good for nanopre reads? (I read that fastQC added support for processing files of Oxford Nanopore reads), Which other tools are recommended for quality control checks on nanopore sequencing reads?

Thanks

Assembly sequencing • 16k views
ADD COMMENT
0
Entering edit mode

Thanks a lot for the reply

ADD REPLY
4
Entering edit mode
6.6 years ago
arfesta ▴ 40

Here are a few tools I've used..I think the question comes down to what exactly do you mean by QC (what stats are you trying to find out):

minion_qc

IONiser

Nanoplot..as seen here on biostars

ADD COMMENT
0
Entering edit mode
6.6 years ago
GenoMax 141k

See posts #358-359 in this thread for a possible solution.

ADD COMMENT

Login before adding your answer.

Traffic: 2706 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6