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6.5 years ago
snishtala03
▴
70
Hello,
I have fasta sequences of 7 viral RNA transcripts. I need to see if I can recover the correct counts of these transcripts.
I am guessing I will first have to use a software ( like polyester or rsem) to generate simulated fastq files for these transcripts and then use a transcript assemble software (like trinity or stringTie) to quantify them.
Please suggest if this is the right way of doing it and tools I can use to get my results?
Thank you for your help!