Hello, I am doing 16s rRNA analysis on Ion Torrent data using Usearch pipeline. I got error at quality filtering step. Command used for the quality filtering is:- usearch -fastq_filter Reads_trunc.fq -fastq_maxee 1.0 -fastaout Reads_filtered.fa
Error got is:- "CharToIntQual('L') Phred score 43 out of range 0..41"
I have changed the -fastq_maxee value to 0.5 but still got the same error. I can't figure out the reason behind the error. Need help
Thanks in advance.