For an RNAseq time points data - control, 1, 2 , 4 days I separately did pathway analysis and has pathway enriched along with -log10 (p) in the form of bar diagrams. I have selected some pathways that are common in at least two time points while there are few pathways that are only in last time point and has biological significance. What should be the best way to show if there is any significant enrichment over time. CAn we perform some sort of statistical test like z score (like IPA does for canonical pathways) or we just show it bar diagrams at different time points with p value. Any suggestion.
Sankey plots are great for showing how sets and subsets of sets merge, diverge, grow and shrink over time.
You could use relative ribbon width and color attributes to communicate quantity and categorization of genes or other data that change over time.