Question: Normalisation of Microbiome Data for Beta Diversity Analysis
gravatar for qwrissie
2.5 years ago by
qwrissie0 wrote:

I am looking for methods to normalise the read counts in an OTU table prior to beta-diversity analyses and hierarchical clustering of samples. I want to avoid rarefying because some samples have very low reads counts and this would therefore greatly increase error. Converting to a percentage would ignore the differences in variance between samples. Can anyone recommend any other methods?

ADD COMMENTlink modified 2.5 years ago by Biostar ♦♦ 20 • written 2.5 years ago by qwrissie0

Have you tried phyloseq for the same purpose?

ADD REPLYlink written 2.5 years ago by sridhar56100
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