Question: How can we calculate expression value from raw data
gravatar for lmanohara99
24 months ago by
Sri Lanka
lmanohara9920 wrote:

In NCBI GEO website, We can find the expression dataset sample. I need to know how this expression value is calculated based on the raw data. In other words, What methods are used to transform raw values to a single expression value? I appreciate your help to figure out the sample dataset which I have been referring here.

gsea rna-seq sequence • 669 views
ADD COMMENTlink modified 24 months ago by Michael Dondrup46k • written 24 months ago by lmanohara9920
gravatar for Michael Dondrup
24 months ago by
Bergen, Norway
Michael Dondrup46k wrote:

That depends from dataset to dataset, however, if you read the content of the page you are linking to, you will find the description:

Data processing The scanned images were analyzed with Feature Extraction Software (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. The intensity signals was imported to CLC Main workbench 6.0 and normalized by the quartile normalization method and log2 transformed.

ADD COMMENTlink written 24 months ago by Michael Dondrup46k
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