running DESEQ2 results in GSEA
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6.6 years ago

I am trying to run my preranked rnk file (generated using the DESEQ results). I followed the instructions given in http://genomespot.blogspot.com/2014/09/data-analysis-step-8-pathway-analysis.html to generate it.

When I run the rnk file GSEA, I get the following error:

"After pruning, none of the genes passed size thresholds."

How do I troubleshoot this?

RNA-Seq • 3.2k views
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It will be easy if you can provide the parameters used for the analysis.

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