Question: normalization constant in GFOLD
19 months ago by
ashish • 200
ashish • 200 wrote:
I am trying GFOLD on an unreplicated RNA-seq data sets. I have two queries regarding it.
- what is the normalization constant and what does the values signify?
- In one of the datasets I recieved a warning saying the median of gene read count is zero. What does this mean?
-VL1 Calculating normalization constant ... -VL1 WARNING: The median of gene read count is zero. The normalization method proposed by DESeq cannot be used. Use total read count to do normalization. -VL1 Normalization constant is: mouse_15 6.04383e+07 2.61471 mouse_17 2.31147e+07 1 -VL1 Calculate RPKM ...
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