I am trying to understand the theoretical bases and practical causes for why R2 is poorer quality compared to R1 for Illumina Paired End sequencing.
While there are some posts here that discuss this observation: MiSeq: R1 vs. R2 reads show big quality difference - is that normal? Should I trim both R1 and R2 if only R2's quality is poor?
what I am most interested is in understanding WHY this happens. Could someone point me in the right direction please - specific papers, or an Illumina technical note, a Youtube presentation, etc. - anything useful. THANK YOU!