I have 80 DNA samples in which we have sequences three overlapping sections of a large gene using the sanger method. I am now looking for a way to merge these sequenced segments of the gene into a single sequence for each sample so that they can be aligned for analysis.
I have come across a couple of tools for doing this with just two overlapping sequences (such as emboss), and i've seen that this can be done with bioedit for one sample at a time, but is there a tool that can allow me to do this in bulk. or will i have to align and assemble them as i would with ngs data of a genome?
Thankful for any answers.
Kind Regards, Tom