I recently done small RNA sequencing of my endocrine tumour samples. I got result analysis with Mirdeep2 . I am not master in bioinformatics so , i outsourced the analysis. The company have predicted some novel micrornas. They have used mirdeep2 software for predicting novel microRNA.
I am facing problem in selecting novel microRNAs from small RNA sequencing. Is it necessary to have high mirdeep2 score with high mature read count. Those sequence with high mirdeep2 score ( like 3168) & mature read count (456) have non significant RANDFOLD p value. I am confused in this part.
I read in mirdeep2 documentation in which the score cut was from -10 to 10.but i am getting mirdeep2 score of like 3168 - 0. I am really very confused. Then i started comparing total read length , loop read length. But still confused.
Please guide me regarding mirdeep2 software and please help me its parameters for selecting novel microRNA.I thanks.