While analyzing my microarray data, I looked at the images of the chips. It has an empty band on the right side and a spot in the middle - photos of the first array are attached bellow. The same pattern is present on all (16) microarrays in my experiment. Raw image: PLM image: I dont have any experiance looking at chip images, so the band looks confusing to me. Is that normal or concerning? Anyone has an explanation?
Thanks in advance!