Reading splice daa

Question

[oatgoat]

New to this field. I have a dataset where the mutation type is splice and the alteration is coded "c.673-3T>G". How to interpret this? How do i translate this into what protein change has occoured ie what codon? Thanks

[Nicolas Rosewick]

do you have access to the associated RNA-Seq ? If yes first take a look in IGV

Answer 1

[Devon Ryan]

If this is a change in a splice site then there may not be a change in the protein sequence at all, there might simply be no protein. Or maybe there's less protein. Or maybe there's a switch in the isoform usage.