Question: Reading splice daa
gravatar for oatgoat
11 days ago by
oatgoat0 wrote:

New to this field. I have a dataset where the mutation type is splice and the alteration is coded "c.673-3T>G". How to interpret this? How do i translate this into what protein change has occoured ie what codon? Thanks

next-gen • 99 views
ADD COMMENTlink modified 11 days ago by Devon Ryan71k • written 11 days ago by oatgoat0

do you have access to the associated RNA-Seq ? If yes first take a look in IGV

ADD REPLYlink written 10 days ago by Nicolas Rosewick5.5k
gravatar for Devon Ryan
11 days ago by
Devon Ryan71k
Freiburg, Germany
Devon Ryan71k wrote:

If this is a change in a splice site then there may not be a change in the protein sequence at all, there might simply be no protein. Or maybe there's less protein. Or maybe there's a switch in the isoform usage.

ADD COMMENTlink written 11 days ago by Devon Ryan71k
Please log in to add an answer.


Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1406 users visited in the last hour