Question: Are regular illumina poly(a) rna-seq data suitable to study polyadenylation sites?
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gravatar for Alexie Li
7 days ago by
Alexie Li20
Alexie Li20 wrote:

Hi all!

I have a question about is there a particular requirement for RNA-seq to study poly(A) in a species? I have many rna-seq data using poly(A) based library preparation method, but they are not designed to study alternative polyadenylation problem. From my understanding, the data should harbor all poly(A) information, but I am not sure whether I need an AT-rich designed RNA-seq?

Thank you in advance!

rna-seq poly(a) sites • 87 views
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