Question: Lnc RNA,RNA Seq
1
gravatar for shafnaasmy.v.s
18 months ago by
shafnaasmy.v.s20 wrote:

hi

I have a RNA seq data and planning to do LNC rna analysis.

LNC Rna analysis can be done only on small RNA seq or can be done using Rna seq data?

lncrna rna-seq • 653 views
ADD COMMENTlink modified 18 months ago by Devon Ryan89k • written 18 months ago by shafnaasmy.v.s20
1
gravatar for Devon Ryan
18 months ago by
Devon Ryan89k
Freiburg, Germany
Devon Ryan89k wrote:

The l in lncRNAs stands for long, which in this case is >200 bases. Given that, an sRNAseq library prep would get rid of most lncRNAs. You need a ribo-depleted RNAseq dataset (the typical prep uses polyA selection, which will exclude some non-coding RNAs).

ADD COMMENTlink written 18 months ago by Devon Ryan89k

hi

thank you for helping me out.i am working on already available RNA seq data from SRA. So how do i find its a ribo-depleted RNA seq.

ADD REPLYlink written 17 months ago by shafnaasmy.v.s20

You'd have to look at whatever metadata is provided, usually this is mentioned somewhere in there.

ADD REPLYlink written 17 months ago by Devon Ryan89k
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