Hi, all =)
I'm trying to use de DISOPRED3 program, but this error appears:
$ ../run_disopred.pl ../examples/example.fasta Running PSI-BLAST search ... [../run_disopred.pl] ERROR: /home/eac16/local/ncbi/blast/legacy/2.2.26/bin/blastpgp -i /home/eac16/local/DISOPRED/3.16/examples/example.fasta -d /scratch/eac16/dbs/blast/legacy/uniprot/uniref90 -a 2 -b 0 -j 3 -h 0.001 -C /home/eac16/local/DISOPRED/3.16/examples/example_16870_8d0a0101.chk -o /home/eac16/local/DISOPRED/3.16/examples/example_16870_8d0a0101.blast **failed: 256**
The DISOPRED3 uses the legacy blast, so I used the command "formatdb" (2.2.26 version ) to create the database.
$ formatdb -i uniref90.fasta -p T -o T
The paths are correct and the variables at the run_disopred.pl's script too:
my $NCBI_DIR = "/home/eac16/local/ncbi/blast/legacy/2.2.26/bin/"; my $SEQ_DB = "/scratch/eac16/dbs/blast/legacy/uniprot/uniref90"; my $PSIBLAST_PAR = "-a 1 -b 0 -j 3 -h 0.001";
I installed and used the DISOPRED3 in a HPC once and use to predict (proteins) an entire genome and it was done. But now I moved to another lab, and I don't know what is wrong with the software/instalation.
Can someone help me?