Entering edit mode
6.5 years ago
andremrsantos
•
0
Hi everyone,
I've been trying to run SNAP-aligner (http://snap.cs.berkeley.edu/) to process some SRA samples. But I found some troubles when running it.
I decompressed SRA into gzip fastq files and runned the following command:
$ snap-aligner paired ~/rsc/hg38_snap \
../raw/ERR1019070_1.fastq.gz \
../raw/ERR1019070_2.fastq.gz \
-o ${NAME}.bam -F s -so
And the program output the following:
Welcome to SNAP version 1.0beta.18.
Loading index from directory... 433s. 3219030417 bases, seed size 20
Aligning.
FASTQ record larger than buffer size at ../raw/ERR1019070_2.fastq.gz:14632868274
SNAP exited with exit code 1 from line 255 of file SNAPLib/FASTQ.cpp
FASTQ record larger than buffer size at ../raw/ERR1019070_1.fastq.gz:14934082722
SNAP exited with exit code 1 from line 255 of file SNAPLib/FASTQ.cpp
I am not sure what happend and the fastq files seem fine. Do you guys have any ideas or suggestions?