Whole-transcriptome RNASeq data of RNA-i engineered organism
0
0
Entering edit mode
4.0 years ago
Macspider ★ 3.4k

I am working with an organism that has been engineered with a transgene to perform RNA-interference. I have whole-transcriptome RNASeq data for this organism. Now:

  • I expect RNA reads coming from the transgene in my sample
  • I also expect RNA reads from the downregulated transcript, but less than the wild type

The sequencing is illumina sequencing, 125 nt read length, 2 replicates. In both replicates I find both conditions verified.

I am asking:

  • Do you agree with these two statements?
  • Do you have good literature to cite in support / aganist this?

I am writing a manuscript and need to pile up as much evidence as possible. So far I wasn't able to find a good reference to say that the sequencing can uptake transgenic molecules, and that targeted molecules are still present even if targeted and can occasionally be sequenced.

EDIT: found it! for future readers, this paper is a good literature reference:

http://www.annualreviews.org/doi/full/10.1146/annurev-biophys-083012-130404

RNA-Seq RNAi sequencing transcriptome transgene • 722 views
ADD COMMENT

Login before adding your answer.

Traffic: 2063 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6