please, what is the line command that allows you to convert an illuminated file into fastq snager thanks for your help
Have you tried tools provided here: Illumina-Fastq To Sanger-Fastq Conversion
I want to convert an Illuminat type file to fastq Sanger i need the command line
Please explain what you are trying to achieve and why, so that we can help you, as this request is confusing. Do you have a bcl file?
Repeating the question does not count as explaining. Please elaborate.
Please try the seqtk solution provided on this post: Illumina-Fastq To Sanger-Fastq Conversion
seqtk seq -Q64 -V Read1.fastq > Read1.sanger.fastq
PS: It is good practice to ensure that a similar question doesn't exist or provided solutions don't work before a question is posted here.
You can also use reformat.sh from BBMap suite as follows: reformat.sh in=your_fastq.gz out=sanger_fastq.gz qout=33 (adjust file names as needed, use in1= in2= out1= out2= for PE data.
reformat.sh in=your_fastq.gz out=sanger_fastq.gz qout=33
in1= in2= out1= out2=
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