Question: PINDEL and somatic mutations
0
gravatar for Leandro de Mattos
12 months ago by
Brazil
Leandro de Mattos80 wrote:

For illumina I need to Recalibrated bases and Indelrealigned. I would like to known if Is necessary Recalibrated bases and Indelrealigned (GATK software) of bam (IonTorrent) for further Pindel Analysis?? Thanks, Leandro

pindel indelrealigned gatk • 503 views
ADD COMMENTlink modified 3 months ago by Kevin Blighe32k • written 12 months ago by Leandro de Mattos80
0
gravatar for Kevin Blighe
3 months ago by
Kevin Blighe32k
Republic of Ireland
Kevin Blighe32k wrote:

Oi Leandro,

Não, não e necessário. Você só tem que mapear os seus reads à genoma da referencia, como hg38.fasta e com, per exemplo, bwa mem (se os seus reads tem um comprimento de >70bp).

Obrigado / Abraços, Kevin


Translation: [Hey Leandro, No, it's not necessary..You just have to align your reads to the reference genome, like hg38.fasta, and with, for example, bwa mem (if the reads have length >70bp). Thanks / Hugs, Kevin]

ADD COMMENTlink modified 3 months ago • written 3 months ago by Kevin Blighe32k
1

Eu que agradeço Kevin. Abs

Em terça-feira, 17 de julho de 2018, Kevin Blighe on Biostar < mailer@biostars.org> escreveu:

ADD REPLYlink written 3 months ago by Leandro de Mattos80
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