Duplication level (RNA)

Question

[Mozart]

Hello there, I'm trying to figure out how to evaluate this graph. ![enter image description here][1]

Since I am a newbie I would like to understand a bit more the meaning of the two lines and their trends. Basically, I supposed that about 50 percent of the sequences are not duplicated with another peak at more than 10. but why there are 2 lines?

thanks

Answer 1

[GenoMax]

Take a look at this blog post by Dr. Simon Andrews, author of FastQC.