Question: node degree in cytoscape to find hub proteins
gravatar for rajasekargutha
2.3 years ago by
rajasekargutha30 wrote:

Hi, I am working on protein interaction network using cytoscape. Once I prepare the network, and use some Network Visualizer ( like Cytoscape), I can get some network features. I wanted to know about the significance of these network values like node degree and centrality measures such as Betweenness Centrality, Closeness Centrality, etc. Also, How to decide the hub proteins with these values.

I hope my question is not be ambiguous.

ADD COMMENTlink modified 2.3 years ago by Diwan570 • written 2.3 years ago by rajasekargutha30
gravatar for Diwan
2.3 years ago by
United States
Diwan570 wrote:

In cytoscape, you can use "network analyzer" which will be available as default and run "analyze network". It will calculate all properties of network. Hubs are the nodes with higher (more) degree i.e., nodes with more connections. Just sort the output list by degree and you can identify hub nodes. Betweeness centrality (communication hot spots) are nodes that connect distinct parts of the network.

Check this:

You might find the "PPI network analysis" part of this paper useful:

ADD COMMENTlink written 2.3 years ago by Diwan570

How to colour only hub protein nodes with different colour

ADD REPLYlink written 2.3 years ago by rajasekargutha30

Just add a new node property and fill it with 1's and 0's, i.e., put hub nodes as 1's. Then in cytoscape, viewer panel, you can color according to this property.

If there are few nodes, just select them manually and override default color for that hub node only. HTH

Another way to highlight a hub is to alter node size and make them bigger than rest of other nodes.

ADD REPLYlink written 2.3 years ago by Diwan570

Good answer by Diwan. A colleague of mine has recently been using Network Analyzer in order to derive vertex/node degree, BC, and other metrics.

ADD REPLYlink written 2.1 years ago by Kevin Blighe54k
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