I'm trying to find how to look for a particular isoform in RNA-seq data. In fact I'm interested in a protein that has two isoforms.
I am wondering if one of the isoform in particular is translated in mRNA in human ES cells.
How can I look for that ? I'm new to the RNA-seq analysis and I'm not sure how to proceed... I found RNA-Seq BAM data for hES but I don't know what to do from there.
Any advice would be greatly appreciated !