What is the best method to submit prokaryotic genome assemblies along with annotations (mainly the predicted genes, tRNA and rRNA) into NCBI?
I am currently looking at this guideline from NCBI however it is quite confusing. Does someone have a better idea?
Blast2GO also offers preparation of GenBank submission file however it only allows submission of gene (GFF format).
What I have with me is:
- Genome assembly in fasta format - pacbio platform , assembled with canu assembler.
- Genes predicted by prodigal - gff format and the corresponding proteins/genes in fasta format.
- Predicted tRNA, using tRNAScan- locations in bed format.
- Predicted rRMA, using RNAmmer- locations in GFF format along with sequences in fasta format.