Entering edit mode
6.5 years ago
from the mountains
▴
230
I hope all is well. I have a quick question about my STAR -> stringtie -> cuffcompare workflow. I found it odd that there were no frags of type "j" when I typed the following:
$ cut -f4 ${sample}.tracking | sort | uniq -c
3864 =
14532 c
27422 e
25872 i
8018 o
2031 p
21619 u
19 x
Has anyone encountered this before? It seems very odd that there are 0 novel isoforms. I am using the Rnor_6.0 gtf file, which contains splicing annotations.
Your help would be appreciated. Thanks!
