Hi All, I am looking at this RNA-seq data here. I want to know which specific run do I need to select from as there are 36 different runs starting from SRR id SRR3285884 to SRR3285919. I want to assemble this specific transcriptome data using Trinity for my research and I am not sure which run I should be selecting. Please clarify. Another question I have is about trimming- since the Design description (copied below) from the link above states that the mRNA were selected using poly-T containing beads, Do I need to do the trimming of poly-T/A tails before assembly? Thank you.
Design: RNA-seq libraries have been prepared according to Illumina’s protocols using the Illumina TruSeq RNA sample prep kit to analyze mRNA v2 (P.N. RS-122-2001) to analyze mRNA. mRNA were selected using poly-T containing beads to remove other RNA. Then, RNA were fragmented to generate double stranded cDNA to be sequenced.