I am not a molecular biologist and I am mostly doing RNA-Seq. I want to find marker genes based on RNA-Seq results and I have to discuss with people at the bench about candidate genes would be good markers. These genes should thus have a measurable expression using qPCR.
I am wondering what would be the minimal TPM I should take to be able to detect the expression of a gene using qPCR.
In this paper it is written:
First, genes were filtered based on a minimal expression of 0.1 TPM in all samples and replicates, to avoid the bias for low expressed genes.
Does this mean that all genes with a TPM over 0.1 would be detected by qPCR? Does anyone have the experience of crossing RNA-Seq and qPCR results?
PS: My TPM values are coming from the
tximport function after